Stable immobilized hapten reagent for use in heterogeneous...

Details Stable immobilized hapten reagent for use in heterogeneous... Stable immobilized hapten reagent for use in heterogeneous...

G - Physics

01

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167/44

G01N 33/547 (2006.01) G01N 33/543 (2006.01) G01N 33/548 (2006.01) G01N 33/72 (2006.01) G01N 33/96 (2006.01)

Patent

CA 1288691

ABSTRACT OF THE DISCLOSURE An immobilized hapten reagent for use in a specific binding assay for the determination of a hapten or binding analog thereof in a liquid test sample and methods for preparing and using the immobilized hapten reagent. The immobilized hapten reagent comprises a hapten moiety covalently linked substantially only to the external surface of a polyacrylamide gel particle . The immobilized hapten reagent is prepared by forming a reaction mixture comprising the hapten moiety and the carrier material in a nonswelling solvent wherein the gel particle is substantially impervious to the hapten moiety and wherein an analytically insignificant amount of the hapten moiety is nonspecifically bound to the gel particle. The immobilized hapten reagent is characterized by being substantially stable in aqueous solutions and exhibits an insignificant amount of leakage of the hapten moiety into a liquid test medium.

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