A - Human Necessities – 61 – F
Patent
A - Human Necessities
61
F
62/1
A61F 7/10 (2006.01)
Patent
CA 1239289
METHOD AND APPARATUS FOR CRYOPREPARING BIOLOGICAL TISSUE FOR ULTRASTRUCTURAL ANALYSIS ABSTRACT OF THE INVENTION This invention relates to a method for the cryo- preparation of biological tissue samples for ultrastruc- tural analysis. The process comprises vitrifying a biological tissue sample under severely rapid conditions and then depressurized. The depresssurized, vitrified tissue sample is brought to equilibrium at a temperature of less than -140°C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is infiltrated with a degassed resin and followed by a polymerization of the resin to form an embedded tissue sample. This invention additionally relates to a sample holder apparatus which has unique use characteristics in the process of this invention.
460541
Meredith & Finlayson
The Board Of Regents The University Of Texas System
LandOfFree
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