Micropropagation process

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47/4, 71/8.3

A01G 7/00 (2006.01) A01H 4/00 (2006.01) A01N 43/653 (2006.01)

Patent

CA 1297694

ABSTRACT Z/PP 33948 "MICROPROPAGATION PROCESS" The viability of plantlets on transfer from in vitro micropropagation conditions to in vivo conditions is improved by incorporating in the in vitro medium from 0.001 to 10 mg of a plant growth regulator per litre of in vitro medium wherein the plant growth regulator is an inhibitor of the gibberellin pathway. Preferred plant growth regulators are kaurene oxidase inhibitors, for example paclobutrazol, (2RS, 3RS)-1-(4-chlorophenyl)-4,4- dimethyl-2-(1H-1,2,4-triazol-1-yl)pentan-3-ol. The plantlets are preferably cultured in contact with a solid porous carrier housed in a liquid in vitro culture medium.

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