Bacterial methionine n-terminal peptidase

Details Bacterial methionine n-terminal peptidase Bacterial methionine n-terminal peptidase

C - Chemistry, Metallurgy

12

N

530/13, 167/139,

C12N 9/52 (2006.01) C07H 21/04 (2006.01) C07K 14/415 (2006.01) C07K 14/55 (2006.01) C07K 16/40 (2006.01) C12N 1/21 (2006.01) C12N 9/48 (2006.01) C12N 15/57 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01)

Patent

CA 1338642

Methods of obtaining N-terminal methionine-free proteins are described. The methods employ a novel en- zyme, E. coli methionine aminopeptidase either in vitro or in vivo. For in vivo application, plasmid-borne DNA encoding the peptidase is transformed into a bacterial host which produces the desired protein.

516905

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