Method for the construction of randomized gene sequence...

Details Method for the construction of randomized gene sequence... Method for the construction of randomized gene sequence...

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C12N 15/63 (2006.01) C40B 50/06 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2521382

An in vivo method for the construction of randomized gene libraries and/or domain replacement in gene libraries by homologous recombination using a Kluyveromyces lactis killer toxin, in particular the (.gamma.-subunit of the K. lactis killer toxin, as negative selection marker is described. The use of the (.gamma.-subunit of K. lactis as negative selectable marker increases the percentage of randomized clones.

L'invention concerne un procédé in vivo pour la réalisation de génothèques randomisées et/ou le remplacement de régions dans des génothèques par recombinaison homologue à l'aide d'une toxine tueuse appelée Kluyveromyces lactis, notamment la sous-unité .gamma. de cette dernière, comme marqueur sélectif négatif. L'emploi de ladite sous-unité comme marqueur sélectif négatif permet d'augmenter le pourcentage de clones randomisés.

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