Method of producing f(ab')2 fragments of immunoglobulins

Details Method of producing f(ab')2 fragments of immunoglobulins Method of producing f(ab')2 fragments of immunoglobulins

C - Chemistry, Metallurgy

12

P

C12P 21/08 (2006.01) C07K 1/12 (2006.01) C07K 16/06 (2006.01) C07K 16/18 (2006.01)

Patent

CA 2101337

2101337 9213876 PCTABS00014 In the field of monoclonal antibody production, the invention relates to the production of F(ab')2 and Fab' fragments from immunoglobulins. The fragments are produced by acidifying a hybridoma conditioned culture medium containing immunoglobulins with citric acid to a pH of about 3.5 and incubating the conditioned medium at 25 ·C. A proteolytic enzyme in the conditioned medium cleaves the immunoglobulins to F(ab')2 fragments, which may then be treated with a reducing agent to generate Fab' fragments.

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