Uncoupling of dna propagation and expression of protein for...

Details Uncoupling of dna propagation and expression of protein for... Uncoupling of dna propagation and expression of protein for...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 7/01 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2493716

The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.

La présente invention constitue une avancée dans le domaine de la technologie d'expression phagique dans la mesure où elle permet le découplage de la propagation de phages contenant des séquences insérées qui codent des polypeptides hétérologues à partir de l'expression de ces polypeptides. En outre, l'invention concerne des constructions phagiques et leurs procédés d'utilisation pour permettre l'expression d'une protéine de coque phagique, et par conséquent une propagation de phages en l'absence d'une expression de polypeptides hétérologues qui peuvent être exprimés, de manière régulée, sous forme de protéine de fusion avec ladite protéine de coque phagique.

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