C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/21 (2006.01) C12Q 1/18 (2006.01) C12Q 1/32 (2006.01)
Patent
CA 2238862
There is disclosed an assay method of screening and identification of anti- amebic drugs which utilizes the ability to inhibit anaerobic growth of a novel bacterial mutant that expresses the EhADH2 gene and which bypasses the conventional need for a parasitic culture. The novel mutant, designated E. coli/EhADH2, is cultured under anaerobic conditions, a predetermined or known quantity of the agent to be tested or target compound is combined with the cell culture, and the combination is then monitored to determine the inhibitory effect upon the anaerobic growth of the E. coli/EhADH2 cell mutant.
L'invention porte sur une technique analytique de criblage et d'identification de médicaments anti-amibiens faisant, d'une part, intervenir l'aptitude à inhiber la croissance anaérobie d'une nouvelle bactérie mutante qui exprime le gène EhADH2, et évitant, d'autre part, d'avoir à procéder aux habituelles cultures de parasite. Le nouveau mutant, dénommé Escherichia coli/EhADH2, est cultivé en milieu anaérobie et une quantité prédéterminée ou connue de l'agent à essayer ou du composé cible est associée à la culture cellulaire; cette combinaison est ensuite surveillée afin de vérifier l'effet inhibiteur sur la croissance anaérobie de la cellule mutante Escherichia coli/EhADH2.
Osler Hoskin & Harcourt Llp
Washington University
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