Hybrid and single chain meganucleases and use thereof

Details Hybrid and single chain meganucleases and use thereof Hybrid and single chain meganucleases and use thereof

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) A61K 48/00 (2006.01) C12N 15/10 (2006.01) C12N 15/55 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2479153

This patent application relates to hybrid and/or single-chain rare-cutting endonucleases, called meganucleases, which recognize and cleave a specific nucleotide sequence, to polynucleotide sequences encoding for said rare- cutting endonucleases, to a vector comprising one of said polynucleotide sequences, to a cell or animal non-human comprising one of said polynucleotide sequences or said rare-cutting endonucleases, to a process for producing one of said rare-cutting endonucleases and any use of the disclosed products and methods. More particularly, this invention contemplates any use of such rare- cutting endonuclease for genetic engineering and gene therapy.

La présente invention concerne des endonucléases rares hybrides et/ou monocaténaires, appelées méganucléases, qui reconnaissent et coupent une séquence nucléotidique spécifique, des séquences polynucléotidiques codant pour ces endonucléases rares, un vecteur comprenant l'une desdites séquences polynucléotidiques, une cellule ou un animal non humain comprenant l'une de ces séquences polynucléotidiques ou de ces endonucléases rares, un procédé de production de l'une desdites endonucléases rares, et une utilisation quelconque des produits et méthodes présentés dans la description. Plus particulièrement, l'invention se rapporte à une utilisation quelconque de ces endonucléases rares dans le génie génétique et la thérapie génique.

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