Microchip matrix device for duplicating and characterizing...

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 60/00 (2006.01) C12M 1/40 (2006.01) C12Q 1/68 (2006.01) C40B 60/12 (2006.01) G01N 33/53 (2006.01) G01N 33/552 (2006.01) G01N 33/68 (2006.01) G01N 21/03 (2006.01) G01N 21/82 (2006.01)

Patent

CA 2379125

The aim of the invention is to provide a device for duplicating and characterizing nucleic acids almost simultaneously and with a high sample through-put rate, hereby avoiding the disadvantages of the prior art. To this end, the inventive device consists of a chamber body (1) with a recess whose edge sealingly holds an optically transparent chip (2). Said chip holds nucleic acids in individual spots (13) on a detection surface (12). The chamber body (1) is placed on an optically transparent chamber support (5) with a bearing surface (4), in such a way that a capillary gap (7), which can be filled with a liquid sample, is formed between the detection surface (12) of the chip (2) facing towards the chamber support (5) and said chamber support (5). The chamber body (1) is provided with an inlet (81) and an outlet (82), which are spatially separate from each other, and has a space, which laterally encompasses the chip (2) and which has a gas reservoir (6). The chamber support (5) is provided with heating elements.

L'invention concerne un dispositif d'amplification et de caractérisation d'acides nucléiques qui permette une amplification et une caractérisation quasiment simultanées avec un rendement de prélèvement élevé, ce qui permet de remédier aux inconvénients de l'état de la technique. Ce dispositif est constitué d'un corps chambre (1) qui présente un évidement dont la bordure porte de façon hermétique une puce (2) optiquement transparente. Cette puce (2) porte des acides nucléiques à différents endroits (13) d'une face de détection (12). De plus, le corps chambre (1) est posé, par une surface de pose (4), de façon hermétique sur un support de chambre (5) optiquement transparent, de telle façon qu'une fente capillaire (7), pouvant être remplie d'un liquide de prélèvement, est formée entre la face de détection (12) de la puce (2), face tournée vers le support de chambre (5), et le support de chambre (5). Le corps de chambre (1) est doté d'une entrée (81) et d'une sortie (82) spatialement séparées l'une de l'autre. Le corps de chambre (1) présente un espace englobant latéralement la puce (2) et entourant un réservoir de gaz (6). Le support de chambre (5) est pourvu de moyens thermiques.

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