Methods and kits for multiplex amplification of short tandem...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2704463

Methods and materials are disclosed for use in simultaneously amplifying at least 11 specific STR loci of genomic DNA in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of 16 specific loci in a single multiplex reaction, comprising the 10 AmpFISTR? SGMplus? STR loci, the Amelogenin locus, and 5 new STR loci, including methods and materials for the analysis of these loci.

La présente invention concerne des procédés et des substances destinés à une utilisation dans l'amplification simultanée d'au moins 11 loci de séquences courtes répétées en tandem (STR) spécifiques de l'ADN génomique dans une unique réaction multiplexée. L'invention concerne également des procédés et des substances destinés à une utilisation dans l'analyse des produits de telles réactions. La présente invention concerne en outre des substances et des procédés destinés à l'amplification simultanée de 16 loci spécifiques en une unique réaction multiplexée, comprenant les 10 loci de STR AmpFlSTR® SGMplus®, le locus de l'amélogénine, et 5 nouveaux loci de STR. L'invention concerne également des procédés et des substances destinés à l'analyse de ces loci.

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