Metal ion determination by sandwich aggregation assay

Details Metal ion determination by sandwich aggregation assay Metal ion determination by sandwich aggregation assay

G - Physics

01

N

G01N 33/20 (2006.01) G01N 21/31 (2006.01) G01N 21/47 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/84 (2006.01)

Patent

CA 2185777

The present invention relates to a method for the determination of polyvalent metal ions using a sandwich aggregation assay. One or more chelating agents capable of forming at least 2:1 or higher ratio stoichiometry complexes with polyvalent metal ions are attached to a suitable carrier such as latex particles through covalent bonds or by non-covalent interactions, such as hydrophobic interactions. Upon complexation with the metal ions, the carriers of the complexing agent aggregate, cause an increase in light absorbance or light scattering which is proportional to the concentration of metal ions in the test sample. The measurement of the light absorbance or light scattering by a suitable instrument, such as a spectrophotometer or nephelometer provides a means for measuring the concentration of metal ions.

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