C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2586246
The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.
L'invention concerne des méthodes et des routines permettant de développer et d'optimiser des dosages de détection d'acide nucléique destinés à être utilisés en recherche fondamentale, en recherche clinique et dans le développement de dosages de détection cliniques. L'invention concerne, en particulier, des méthodes permettant de concevoir des amorces oligonucléotidiques destinées à être utilisées dans des réactions d'amplification multiplex. L'invention concerne également des méthodes permettant d'optimiser des réactions d'amplification multiplex. L'invention concerne enfin des méthodes destinées à des dosages combinés de génération de signal et de cible.
Allawi Hatim T.
Elagin Vecheslav A.
Hall Jeff G.
Law Scott M.
Lyamichev Victor
Smart & Biggar
Third Wave Technologies Inc.
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