Photochemical internalization for virus-mediated molecule...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/87 (2006.01) A61K 41/00 (2006.01) A61K 48/00 (2006.01) C12N 15/86 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2430334

The present invention provides a method for introducing a molecule into the cytosol of a cell in which the cell is contacted with a photosensitising agent, the cell is irradiated with light of a wavelength effective to activate the photosensitising agent and, substantially at the same time or after the irradiation, the cell is contacted with the molecule to be introduced, particularly for use in cancer treatment, gene therapy and vaccination.

L'invention concerne un procédé permettant d'introduire une molécule dans le cytosol d'une cellule. Ce procédé consiste à faire entrer la cellule en contact avec un agent photosensibilisant, puis à irradier la cellule avec une lumière présentant un longueur d'onde permettant d'activer l'agent photosensibilisant, et simultanément à cette irradiation ou après cette dernière, à faire entrer la cellule en contact avec la molécule devant être introduite. Ce procédé convient en particulier pour les traitements anticancéreux, les thérapies géniques et les vaccinations.

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