Microarray method of genotyping multiple samples at multiple...

Details Microarray method of genotyping multiple samples at multiple... Microarray method of genotyping multiple samples at multiple...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2414105

A method for genotyping multiple samples at multiple genetic loci in a single assay is provided. Microarrays of genomic segments representing discrete loci are formed and hybridized with mixtures of synthetic oligonucleotides that are complementary to the genomic segments. Genotyping information is derived by reading the microarray signals. The method can be used to characterize samples from diverse biological sources and for a variety of applications.

Cette invention a trait à une technique de génotypage de plusieurs échantillons sur plusieurs loci génétiques en une seule épreuve biologique. On constitue des micro-réseaux de segments génomiques représentant des loci distincts et on les hybride avec des mélanges d'oligonucléotides de synthèse qui sont complémentaires de ces segments génomiques. La lecture des signaux émanant des micro-réseaux permet de dériver une information de génotypage. Il est possible d'utiliser cette technique pour caractériser des échantillons issus de différentes sources biologiques. Cette technique a, également un large éventail de domaines d'application.

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