C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 5/04 (2006.01) C12N 15/11 (2006.01) C12N 15/80 (2006.01) C12N 15/81 (2006.01) C12N 15/85 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2570165
The present invention relates to a method for expressing a transgene in a host cell that permits transcriptional termination of the transgene to occur without having to rely on a functional termination site in the DNA used for the transformation. Additional 3' regulatory sequences and 3' end processing enhancing sequences and/or structures can be present in the transformation vector or as a fusion with the transgene of interest.
La présente invention concerne une méthode d'expression d'un transgène dans une cellule hôte qui permet à la terminaison transcriptionnelle du transgène de se produire sans avoir recours à un site de terminaison fonctionnel dans l'ADN utilisé pour la transformation. Des séquences régulatrices 3' supplémentaires et des séquences et/ou structures d'amélioration du traitement d'extrémité 3' peuvent être présentes dans le vecteur de transformation ou en tant que fusion avec le transgène d'intérêt.
Brandle James E.
Brown Kirk Devon
Gowling Lafleur Henderson Llp
Her Majesty The Queen In Right Of Canada As Represented By The M
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