Compositions and methods for modulating cell proliferation...

Details Compositions and methods for modulating cell proliferation... Compositions and methods for modulating cell proliferation...

C - Chemistry, Metallurgy

12

N

C12N 9/96 (2006.01) C07K 14/475 (2006.01) C07K 14/52 (2006.01) C07K 14/71 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01)

Patent

CA 2243300

Methods and compositions are provided for in vitro expansion of growth factor dependent cells. Expansion is effected through the use of growth factor conjugates that include a growth factor such as a steel factor and a polysaccharidase substrate binding region. The conjugates are immobilized by binding of the substrate binding region to a substrate of the polysaccharidase in a growth chamber for the cells.

L'invention concerne des procédés et des compositions pour la culture in vitro de cellules nécessitant des facteurs de croissance. La croissance des cellules est assurée par l'utilisation de conjugués de facteurs de croissance tels que la cytokine SLF et d'une région de fixation d'une polysaccharidase à son substrat. Les conjugués sont immobilisés en fixant la région de fixation au substrat à un substrat de la polysaccharidase dans un flacon de culture cellulaire.

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