Method of increasing recovery of heterologous active enzymes...

Details Method of increasing recovery of heterologous active enzymes... Method of increasing recovery of heterologous active enzymes...

C - Chemistry, Metallurgy

12

N

C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/16 (2006.01) C12N 9/96 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2412447

A method of increasing recovery of active enzyme produced in a plant is provided where the enzyme requires a transitional metal cofactor for activation. The metal cofactor is supplied to the enzyme during plant development, during extraction, or after extraction. Recovery of active enzyme is also provided by incubating the extracted enzyme at a non-enzyme degrading temperature. Addition of a negative ion salt further improves active enzyme recovery. Optimum salt concentrations for recovery of laccase from plants using copper solutions is provided.

Procédé pour augmenter la récupération de l'enzyme active produite dans une plante, ladite enzyme nécessitant pour l'activation un cofacteur de métal de transition. Le cofacteur de métal est fourni à l'enzyme pendant le développement de l'enzyme ainsi que pendant ou après l'extraction. On effectue également la récupération en incubant l'enzyme extraite à une température qui permet d'éviter la dégradation de l'enzyme. L'ajout d'un sel d'ion négatif augmente davantage la récupération de l'enzyme active. L'invention concerne aussi des concentrations de sel optimales visant la récupération de laccase à partir des plantes au moyen de solutions de cuivre.

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