Vectors for gene mutagenesis and gene discovery by gene...

Details Vectors for gene mutagenesis and gene discovery by gene... Vectors for gene mutagenesis and gene discovery by gene...

C - Chemistry, Metallurgy

12

N

C12N 15/867 (2006.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2351741

Novel vectors are described that incorporate, inter alia, a novel 3' gene trap cassette that does not encode a marker conferring antibiotic resistance and which can be used to efficiently trap, mutagenize, and identify previously unknown cellular genes. Vectors incorporating the presently described 3' gene trap cassette find particular application in gene discovery, and the production of mutated cells and animals.

L'invention concerne de nouveaux vecteurs qui comprennent entre autres une nouvelle cassette-piège à terminaisons 3' qui ne code pas pour un marqueur conférant une résistance aux antibiotiques et qui peut être efficacement utilisée pour piéger, mutagéniser et identifier des gènes cellulaires inconnus auparavant. Les vecteurs incorporant la présente cassette-piège à terminaisons 3' trouvent notamment une application dans la découverte de gènes et la production de cellules et d'animaux mutagénisés.

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