Methods and apparatus for particle introduction and recovery

Details Methods and apparatus for particle introduction and recovery Methods and apparatus for particle introduction and recovery

G - Physics

01

N

G01N 27/00 (2006.01) C12N 15/10 (2006.01) G01N 27/447 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2713313

Particles may be injected into a matrix for concentration by scodaphoresis using a quadrupole injection field. Particles may be injected from two or more sample chambers simultaneously. Particle injection may be performed simultaneously with performing scodaphoresis. In some embodiments the particles are concentrated into a well containing fluid. The well can extend out of a plane of the matrix. Altering the relative phases of components of a scodaphoresis field permits concentration of selected particles and exclusion of other particles. Scodaphoresis methods may be applied to DNA, other bio- molecules and other particles.

Selon l'invention, des particules peuvent être injectées dans une matrice pour concentration par scodaphorèse à l'aide d'un champ d'injection quadripolaire. Des particules peuvent être injectées à partir d'au moins deux chambres d'échantillon simultanément. L'injection de particules peut être effectuée simultanément avec la réalisation d'une scodaphorèse. Dans certains modes de réalisation, les particules sont concentrées dans un puits contenant du fluide. Le puits peut s'étendre hors d'un plan de la matrice. La modification des phases relatives de composants d'un champ de scodaphorèse permet une concentration de particules choisies et l'exclusion d'autres particules. Les procédés de scodaphorèse peuvent être appliqués à l'ADN, d'autres biomolécules et d'autres particules.

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