Microbially expressed xylanases and their use as feed...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) A23K 1/165 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 15/63 (2006.01) C12N 15/81 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2548723

The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

La présente invention concerne des séquences codant pour la xylanase à codon optimisé et l'expression de xylanases dans des microbes et des levures. L'invention concerne également l'utilisation de plusieurs copies de la construction d'expression de la xylanase pour des niveaux élevées d'expression protéique. L'invention concerne également l'utilisation de xylanases comme additifs alimentaires ou comme additifs pour aliments pour animaux. L'invention concerne également des procédés d'expression d'enzymes visant à augmenter la thermorésistance, consistant à exprimer les enzymes dans des organismes qui permettent d'obtenir la glycosylation des protéines, par rapport à l'expression de cette même enzyme sans glycosylation. L'invention concerne également des procédés de préparation d'aliments pour animaux, d'additifs pour aliments pour animaux enzymatiques, ainsi que des procédés visant à réduire l'indice de consommation ou à augmenter la prise de poids des animaux.

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