Substrate trapping protein tyrosine phosphatases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) A61K 38/00 (2006.01) C12N 9/16 (2006.01) C12N 15/11 (2006.01) C12N 15/62 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2375145

Compositions and methods are provided pertaining to novel substrate trapping mutant protein tyrosine phosphatases (PTPs) that are catalytically impaired but which retain the ability to bind phosphotyrosine-containing protein substrate(s), and that are further modified by the replacement of at least one tyrosine residue with an amino acid that cannot be phophorylated. Uses of such PTPs for identification of PTP substrates, and of agents that alter PTP- substrate interactions are disclosed, as are methods of altering PTP activities.

L'invention concerne des compositions et des méthodes applicables à des nouvelles protéine-tyrosine-phosphatases (PTP) mutantes de piégeage de substrats, qui malgré une altération catalytique demeurent capables de lier un ou plusieurs substrats de protéines contenant de la phosphotyrosine, et qui sont également modifiées par remplacement d'au moins un résidu de tyrosine par un amino-acide ne pouvant être phosphorylé. L'invention concerne également l'utilisation de ces PTP pour l'identification de substrats de PTP et d'agents modificateurs des interactions PTP-substrat, ainsi que des méthodes destinées à modifier l'activité des PTP.

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