Method for quantitating a protein by image analysis

G - Physics – 01 – N

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G01N 33/53 (2006.01) G01N 33/50 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2397417

The present invention provides a method for determining expression levels of one or a multiplicity of target proteins in a tissue or cell sample by the use of immunohistochemical staining of target cells and determination of optical density of the cells. The said optical density is compared to a calibration curve generated from control cells whose expression level of the target protein is known.

La présente invention concerne une méthode permettant de déterminer les niveaux d'expression d'une ou de plusieurs protéines cibles dans un échantillon tissulaire ou cellulaire par coloration immunohistochimique de cellules cibles, et détermination de la densité optique desdites cellules. La densité optique est comparée à une courbe d'étalonnage produite à partir de cellules témoin dont le niveau d'expression de la protéine cible est connu.

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