Device for rapid dna sample processing with integrated...

B - Operations – Transporting – 01 – L

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Details

B01L 9/06 (2006.01) B01D 61/18 (2006.01) B01D 61/28 (2006.01) B01D 63/02 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) B01L 3/02 (2006.01) B01L 7/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/34 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2385029

The present invention provides, in one embodiment, a capillary cassette to facilitate rapid dialysis or thermocycling, via liquid or gas, of sub- microliter samples. In one embodiment, the invention is configured for use with a fluid handling machine for automated, high-throughput processing. Dialysis or thermocycling can be performed while the capillary cassette is mounted on the fluid handling machine. However, another embodiment of the invention provides higher throughput by the use of a hotel to simultaneously process a plurality of capillary cassettes. Hotel capabilities include dialysis, thermocycling, cleaning and chemical regeneration. Exemplary applications include but are not limited to polymerase chain reaction (PCR), DNA sequencing applications, oligonucleotide ligation, ligase chain reaction (LCR), single nucleotide extension, exonuclease treatment, and oligonucleotide hybridization assays.

L'invention porte dans une exécution sur une cassette capilaire facilitant des dialyses rapides ou le thermocyclage, via des liquides ou des gaz d'échantillons inférieurs au microlitre. Dans une autre exécution la cassette est configurée pour être utilisée avec une machine de manipulation des fluides permettant un traitement automatique à fort débit, les dyalises et le thermocyclage s'effectuant lorsque la cassette est montée sur ladite machine. D'autres exécutions permettent des débits plus élevés en recourant à des hôtels effectuant le traitement simultané de plusieurs cassettes. Les capacités de ces hotels permettent la dyalise, le thermocyclage, le nettoyage et la régénération chimique. Citons à titre d'exemple des applications englobant non exclusivement la PCR, le séquencage de l'ADN, la ligation des oligonucléotides, la LCR, l'extension de nucléotides isolés, le traitement par l'exonucléase, et les essais d'hybridation d'oligonucléotides.

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