Determination of estradiol by competitive immunoassay

C - Chemistry – Metallurgy – 07 – J

Patent

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C07J 41/00 (2006.01) C12N 9/14 (2006.01) C12N 9/96 (2006.01) G01N 33/53 (2006.01) G01N 33/547 (2006.01) G01N 33/58 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2135887

2135887 9325672 PCTABS00028 The present invention relates to the measurement of estradiol using competitive immunoassay methods. The inventors unexpectedly discovered that estrone and its derivatives conjugated to a label is a particularly effective tracer when used in conjunction with estradiol specific antibodies to determine estradiol levels in fluid samples. The present invention also utilizes 5.alpha.-dihydrotestosterone to enhance the assay performance.

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