Modified inosine monophosphate dehydrogenases

Details Modified inosine monophosphate dehydrogenases Modified inosine monophosphate dehydrogenases

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C07H 21/00 (2006.01) C12N 9/04 (2006.01)

Patent

CA 2491887

The invention relates to a modified IMPDH polypeptide wherein the IMPDH polypeptide has a histidine tag and where the subdomain of the IMPDH polypeptide is modified so that the rate stability of the histidine-tagged, modified IMPDH polypeptide is maintained relative to the wild-type IMPDH polypeptide. The invention also relates to a method, nucleic acid molecules, vectors, and host cells for producing such a histidine-tagged, modified IMPDH polypeptide, and to kits comprising the histidine-tagged, modified IMPDH polypeptide.

L'invention concerne un polypeptide IMPDH modifié qui un tronçon d'histidine et dont le sous-domaine est modifié de sorte que le taux de stabilité du polypeptide du polypeptide IMPDH modifié marqué d'histidine soit maintenu par rapport au polypeptide IMPDH de type sauvage. L'invention vise également une méthode, des molécules d'acide nucléique, des vecteurs et des cellules hôtes pour produire un tel polypeptide IMPDH modifié marqué d'histidine et des ensembles comprenant le polypeptide IMPDH modifié marqué d'histidine.

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