G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01)
Patent
CA 2155318
A method for assaying a specific antibody, comprising the following steps (A), (B) and (C): (A) : forming a complex of a labeled antigen-a specific antibody-a carrier-bound antigen in a first reaction system comprising a mixture of the antigen (labeled antigen) against the specific antibody to be assayed, the antigen being bound to the label in advance, the antigen (carrier-bound antigen) against the above-mentioned specific antibody, the antigen being bound to the carrier in advance, and a liquid sample comprising the above-mentioned specific antibody, (B) : mixing the above-mentioned separated carrier and a labeled antigen same as above after separating the above-mentioned carrier after reaction from the above-mentioned first reaction system to give a second reaction system, and (C) : assaying, after separating the above-mentioned carrier after reaction from the above-mentioned second reaction system, the amount of the label bound to the above-mentioned separated carrier. According to the present invention, a complete complex of carrier-bound antigen-specific antibody-labeled antigen is formed in the first reaction system wherein the two antigens and the specific antibody are reacted by mixing the carrier- bound antigen, the labeled antigen and the liquid sample comprising the specific antibody to be assayed. When an incomplete complex of carrier-bound antigen-specific antibody is present in the second reaction system, a complete complex can be formed by mixing the separated carrier and the labeled antigen after separating the carrier after reaction from the first reaction system. Accordingly, the prozoning phenomenon can be eliminated and the target specific antibody can be assayed with high sensitivity by assaying the amount of the label bound to the carrier, after separating the carrier after reaction from the second reaction system.
Fetherstonhaugh & Co.
Sumitomo Pharmaceuticals Company Limited
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