Micro-array analysis system and method thereof

Details Micro-array analysis system and method thereof Micro-array analysis system and method thereof

G - Physics

01

N

G01N 21/84 (2006.01) C12Q 1/00 (2006.01) C40B 30/00 (2006.01) C40B 60/12 (2006.01) G01N 21/64 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2675103

There is described a system (2) and a method for analyzing spots in a micro-array (5), the spots containing targeted specimen, the method comprising the steps of: providing a slide with a micro-array of the spots thereon; illuminating at least one of the spots (500, 501); directing the light onto the at least one spot; collecting light emitted from the at least one spot; forming an image of the at least one spot using the collected light (502); and analyzing the image to distinguish at least one unit of the targeted specimen located within the at least one spot from any undesired material (506, 507). The analysis results are aimed at the detection, classification and quantification of optically resolvable specimen such as micro-organisms or cells.

La présente invention concerne un système (2) et un procédé permettant d'analyser des points dans un micro-réseau (5), les points contenant un spécimen cible. Ledit procédé comprend les étapes consistant à : disposer d'une lame recouverte d'un micro-réseau de points ; éclairer au moins l'un des points (500, 51) ; diriger la lumière sur ce point ; recueillir la lumière émise par le point ; former une image du point à l'aide de la lumière recueillie (502) ; et analyser l'image pour distinguer au moins une unité du spécimen cible contenu dans le point parmi tout matériau non désiré (506, 507). Les résultats de l'analyse permettent de détecter, classer et quantifier un spécimen à résolution optique tels des micro-organismes ou des cellules.

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