Surfaces coated with streptavindin/avidin

G - Physics – 01 – N

Patent

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Details

G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2294969

The invention relates to surfaces coated with streptavidin/avidin used in medical diagnostics and the pharmaceutical industry. The invention seeks to provide a coated solid phase which has high surface binding yields and sufficient stability, even in the presence of detergents, and which can have universal application and facilitates the necessary sensitivity for the determination procedure to be conducted. The inventive surfaces coated with stretavidin/avidin are characterized in that the coating consists of stretavidin and/or avidin, which is bonded on the surface of a solid supporting material by means of a biotinylated adhering agent.

L'invention concerne des surfaces revêtues de streptavidine/avidine. Les domaines d'application de l'invention sont le diagnostic médical et l'industrie pharmaceutique. Elle a pour objet de mettre au point une phase solide revêtue qui fournit des rendements de fixation élevés sur la surface et présente une stabilité suffisante, même contre les détergents, qui est universellement utilisable et permet la sensibilité nécessaire aux procédés d'analyse à effectuer. Les surfaces revêtues de streptavidine/avidine sont caractérisées en ce que la couche est constituée de streptavidine et/ou d'avidine, lesquelles sont fixées à la surface d'un matériau de support solide par l'intermédiaire d'une substance agrippante biotinylée.

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