C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/06 (2006.01) C07H 21/04 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/22 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G06F 19/00 (2006.01)
Patent
CA 2567624
Translation systems and other compositions including orthogonal aminoacyl tRNA- synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.
L'invention concerne des systèmes de traduction et d'autres compositions comprenant des aminoacyle-ARNt synthétases orthogonales. Ces systèmes de traduction et ce compositions chargent de préférence un ARNt orthogonal avec un acide aminé iodé ou bromé. L'invention concerne des acides nucléiques codant de tels synthétases, ainsi que des méthodes et des trousses pour produire des protéines comprenant des acides aminés contenant des atomes lourds, notamment des acides aminés bromés ou iodés. L'invention concerne des méthodes de détermination de la structure d'une protéine, notamment une protéine dans laquelle un atome lourd a été intégré dans un site spécifique au moyen d'une paire d'ARNt orthogonal/aminoacyle ARNt-synthétase.
Schultz Peter G.
Spraggon Glen
Wang Lei
Wu Ning
Xie Jianming
Fetherstonhaugh & Co.
Irm Llc
The Scripps Research Institute
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