C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/57 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2633661
The invention relates to commercially viable methods for producing biologically active vitamin K dependent pro-teins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epox-ide reductase (VKOR) and vitamin K dependent .gamma.-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
La présente invention concerne des procédés commercialement viables pour produire des protéines dépendantes de la vitamine K biologiquement actives, en particulier le facteur IX. Le facteur IX est produit à un taux d'au moins 15 mg/l et est biologiquement actif à au moins 25 %. Le procédé repose sur la coexpression d'un ou plusieurs parmi l'enzyme de conversion d'acide aminé basique apparié (PACE), l'époxyde réductase dépendante de la vitamine K (VKOR) et la ?-glutamyle carboxylase dépendante de la vitamine K (VKGC) à un rapport préféré de sorte que la protéine dépendante de la vitamine K soit efficacement produite et traitée par une cellule recombinante.
Drohan William N.
Griffith Michael J.
Stafford Darrel W.
Taylor John R.
Inspiration Biopharmaceuticals Inc.
Robic
University Of North Carolina At Chapel Hill
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