Biolistic apparatus for delivering substances into cells and...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/00 (2006.01) A61M 37/00 (2006.01) C12M 3/00 (2006.01) C12N 15/87 (2006.01) F16K 21/00 (2006.01)

Patent

CA 2248622

A biolistic apparatus is provided with a housing having a carrier membrane disposed in a chamber for movement relative to an outlet plate having a plurality of apertures therein. The carrier membrane has a plurality of micro-particles adhered thereto on the surface of the membrane adjacent the apertures for delivery into cells or tissue. A plurality of rupturable membranes are mounted in spaced relation to the carrier membrane. Initially, a vacuum is provided within the assembly including the outlet opening to purge the apparatus of any undesirable gasses. High pressure helium is then delivered to the rupturable membranes and upon reaching a predetermined pressure, will cause the membranes to rupture allowing a shock front of high pressure helium to strike the carrier membrane and impel the articles through the outlet openings.

Appareil de biolistique comportant un boîtier doté d'une membrane porteuse située dans une chambre de manière à se déplacer par rapport à une plaque de sortie comprenant plusieurs orifices. La membrane porte plusieurs micro-particules adhérant à sa surface à proximité des orifices pour être diffusées dans des cellules ou des tissus. Plusieurs membranes rupturables espacées sont fixées à la membrane porteuse. Dans un premier temps, un vide est créé à l'intérieur de l'ensemble, y compris dans l'orifice de sortie, pour faire évacuer tout gaz indésirable de l'appareil. De l'hélium sous haute pression est ensuite envoyé sur les membranes rupturables et, lorsque la pression atteint un niveau prédéterminé, les membranes se rompent, ce qui fait en sorte qu'un front de choc généré par l'hélium sous haute pression est forcé contre la membrane porteuse : les particules sont ainsi éjectées par les orifices de sortie.

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