C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/66 (2006.01) C12N 15/861 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01)
Patent
CA 2383819
Disclosed is a new system for generating recombinant adenovirus vectors and adenovirus-based expression libraries, by positive selection of recombinants deleted for the endogenous protease gene, which gene is expressibly cloned into another region of the adenoviral genome. In a preferred embodiment, the invention allows positive selection of E1-deleted, protease-deleted recombinant adenovirus vectors comprising an exogenous gene or an expressible piece of exogenous DNA, by providing an expression cassette comprising the protease gene and the exogenous DNA inserted in place of E1 region in a shuttle vector. In vivo recombination of the shuttle vector with a protease-deleted adenoviral genome in suitable non-complementing cells generates viable recombinants only when rescuing the protease cloned in E1 region. Non- recombinant viral genomes are not able to grow due to the deletion of the protease gene, ensuring that only recombinant viral plaques are generated. This positive selection can be used for the generation of a large number of high purity recombinant adenovirus vectors and allows generation of adenovirus-based libraries with diversity exceeding 10 6 clones.
Elahi Seyyed Mehdy
Massie Bernard
Oualikene Wahiba
Koenig Hans
National Research Council Of Canada
LandOfFree
Efficient generation of adenovirus-based libraries by... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Efficient generation of adenovirus-based libraries by..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Efficient generation of adenovirus-based libraries by... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1394634