Methods and substances for isolation and detection of small...

Details Methods and substances for isolation and detection of small... Methods and substances for isolation and detection of small...

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C07H 21/02 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C40B 40/08 (2006.01)

Patent

CA 2662837

A capture probe suitable for use with methods for isolating, labeling or detecting small polynucleotides. A method for isolating a small polynucleotide of interest from a sample comprising hybridizing the small polynucleotide to the capture probe and lengthening the small polynucleotide by primer extension or ligation. A method for detecting a small polynucleotide of interest following isolation by amplification of the primer extension products and/or hybridization and subsequent cleavage of dual labeled detector probes.

L'invention concerne une sonde de capture conçue pour être utilisée conjointement à des procédés d'isolement, d'étiquetage ou de détection de petits polynucléotides. L'invention concerne un procédé d'isolement d'un petit polynucléotide à examiner provenant d'un échantillon consistant à effectuer l'hybridation du petit polynucléotide et de la sonde de capture et à prolonger le petit polynucléotide par un prolongement d'amorce ou par une ligature d'amorce. L'invention concerne un procédé de détection d'un petit polynucléotide à examiner qui fait suite à un isolement par amplification des produits de prolongement d'amorce et/ou par hybridation et par clivage subséquent de doubles sondes de détection étiquetées.

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