Thermostable dna polymerase from anaerocellum thermophilum

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2267217

A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophilum. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be negative or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.

L'invention concerne une enzyme thermostable qui est dérivée du microorganisme Anaerocellum thermophilum. Cette enzyme présente un poids moléculaire compris entre 96 et 100 kDa, et exerce une activité de polymérase d'ADN ainsi qu'une activité de transcriptase inverse en présence d'ions de magnésium. Cette enzyme peut être une enzyme native ou de recombinaison. Elle peut être utilisée avec des amorces sélectionnées et des nucléosides triphosphates dans une réaction en chaîne de la polymérase à cycle de température, sur l'ADN ou l'ARN, comme matrice, et avec ou sans polymérases d'ADN supplémentaires comme mélange d'enzyme.

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