C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
233/1, 204/109,
C07K 14/78 (2006.01) A61L 27/24 (2006.01) A61L 27/60 (2006.01) B01J 19/10 (2006.01) C07K 1/14 (2006.01) C08H 1/06 (2006.01) A61F 2/00 (2006.01)
Patent
CA 2025833
ABSTRACT OF THE DISCLOSURE This invention provides a method and the apparatus for producing human autologous collagen from the human fat tissues and specifically for a method of disintegrating fat cells using ultrasonic energy and separating fats, blood, water and serum from the collagen, all of which can be accomplished without removing material from the harvesting syringe. The purpose of the invention is to produce autologous collagen which is useful in several cosmetic procedures used to treat all the problems caused by ageing. In accordance with the procedure taught in the invention after fat cells have been removed from the subject in a harvesting syringe, first the material in the syringe is subjected to an ultrasonic energy source of a range between 20 kHz and 3 mHz which destroys the fat cells by rupturing them which allows the oil in the fat cells to be separated from the residual cell] walls, after which the harvesting syringe is placed in a specially designed centrifuge allowing for the blood, serum, fat cell ail and collagen to be completely separated. In the fat cell disintegration on step, when applying ultrasonic energy, two alternative devices may be employed with the harvesting syringe containing the material. In one, the syringes are placed in a container filled with cold liquid and subjected to ultrasonic energy. The second, preferred method utilizes a high intensity ultrasonic processor that includes a probe that can be placed in the harvesting syringe that is mounted individually in a metal support filled with a cold liquid. In the separation stage, a multi-bodied centrifuge is provided which holds up to four harvesting syringes safely while subjected to centrifugal action to permit the separation of the material as described herein. With the use of the present invention human autologous collagen can be produced in minutes without removal from the frozen storage in an individual patient collagen bank.
Gowling Lafleur Henderson Llp
Zocchi Michele
LandOfFree
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