Mutant luciferases

Details Mutant luciferases Mutant luciferases

C - Chemistry, Metallurgy

12

N

C12N 9/02 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2157476

The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.

L'invention porte sur des mutants actifs non naturels de luciférases de coléoptères et sur l'ADN les codant. Une telle luciférase mutante diffère de la luciférase de type sauvage correspondante en ce qu'elle produit une bioluminescence d'une longueur d'onde dont l'intensité de pointe diffère d'au moins de 1 nm de la longueur d'onde d'intensité de pointe de la bioluminescence produite par l'enzyme de type sauvage. Les luciférases mutantes et les ADN de l'invention servent dans différentes applications de biodétection.

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