Process for producing a target fermentation product

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12P 13/02 (2006.01) C12P 13/04 (2006.01) C12P 17/18 (2006.01) C12P 25/00 (2006.01)

Patent

CA 2353772

The present invention provides a process for producing a target fermentation product. This process includes providing a fermentation medium containing a recombinantly-produced microorganism that over-produces a fermentation product and contains a mutation which causes auxotrophic growth of the microorganism wherein the auxotrophy within the microorganism does not compromise the ability of the microorganism to produce the fermentation product. The medium is then supplied in excess with all substrates required for production of the fermentation product and in growth limiting amounts with a substrate complementing the auxotrophy. Host cells, vectors, and polynucleotide sequences used in the process are also provided.

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