Transformation of sugar cane

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01)

Patent

CA 2746196

Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via Agrobacterium- mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.

La présente invention porte sur des procédés de transformation de la canne à sucre. Les procédés comprennent lutilisation de pousses immatures de canne à sucre comme source de matériau végétal pour la transformation. Des segments de pousse immature sont excisés et transformés au moyen d'un quelconque procédé de transformation approprié. Dans certains modes de réalisation, lesdits segments sont cultivés dans un milieu dinduction de culture embryogénique préalablement à la transformation. La transformation peut être réalisée par le biais de lapport de gène médié par Agrobacterium, de la transformation par biolistique, et similaires. Les plantes transgéniques sont régénérées à partir de plantules cultivées dans des conditions qui favorisent la croissance des cellules transformées et inhibent sensiblement la croissance des cellules non transformées.

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