Ffe media and ffe methods comprising volatile separation media

G - Physics – 01 – N

Patent

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G01N 27/447 (2006.01)

Patent

CA 2690343

The present invention provides methods and separation media for separating analytes of interest via free flow electrophoresis (FFE) using volatile buffer systems. The separation media provided herein allow a convenient separation of the analytes by electrophoresis, and offer the additional advantage that the buffer compounds and the solvent can be easily and residue-free removed after the electrophoretic separation step. Furthermore, methods for mass spectrometric analysis of analytes comprising an FFE method and kits for carrying out FFE separations with volatile buffer systems are also provided. Preferably, the volatile buffer system is TRIS acetate.

La présente invention concerne des procédés et des milieux de séparation pour la séparation d'analytes d'intérêt par électophorèse libre (FFE), qui utilisent des systèmes de tampons volatils. Les milieux de séparation décrits permettent une séparation pratique des analytes par électrophorèse et offrent l'avantage supplémentaire que les composés tampons et le solvant peuvent être éliminés facilement et sans résidus après l'étape de séparation électrophorétique. L'invention concerne également des procédés d'analyse spectrométrique de masse d'analytes qui comprennent un procédé FFE et des kits pour la réalisation de séparations FFE avec des systèmes de tampons volatils. De préférence, le système de tampon volatil est l'acétate TRIS.

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