Automated colorimetric polysaccharide assays

G - Physics – 01 – N

Patent

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G01N 35/02 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2680711

A method of automated determination of saccharide concentration is provided herein. The method includes preparing one or more saccharide standards and one or more diluted polysaccharide test samples, transferring a portion of the one or more saccharide standards and the one or more diluted polysaccharide test samples along with a diluent to a series of wells in a multiwell plate, transferring a portion of an acid reagent to the series of wells in the multiwell plate, mixing the contents of the series of wells in the multiwell plate, heating and cooling the contents of the series of wells in the multiwell plate, shaking the multiwell plate, and measuring the radiant energy absorbance of the contents of the series of wells in the multiwell plate, where all steps are performed in the absence of human intervention.

L'invention concerne un procédé de détermination automatisée de concentration en saccharides. Le procédé comprend la préparation d'un ou plusieurs étalons de saccharide et d'un ou plusieurs échantillons d'essai dilués de polysaccharide, le transfert d'une portion des un ou plusieurs étalons de saccharide et des un ou plusieurs échantillons d'essai dilués de polysaccharide conjointement avec un diluant vers une série de puits dans une plaque multipuits, le transfert d'une portion d'un réactif acide vers la série de puits dans la plaque multipuits, le mélange des contenus de la série de puits dans la plaque multipuits, le chauffage et le refroidissement des contenus de la série de puits dans la plaque multitpuits, le secouement de la plaque multipuits et la mesure de l'absorbance d'énergie rayonnante des contenus de la série de puits dans la plaque multipuits, où toutes les étapes sont effectuées en l'absence d'intervention humaine.

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