Methods for increasing starch content in plant cobs

C - Chemistry – Metallurgy – 12 – N

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C12N 15/113 (2010.01) A01H 5/00 (2006.01) A23K 1/12 (2006.01) A23K 1/14 (2006.01) C12N 15/00 (2006.01) C12N 15/82 (2006.01) C12P 7/06 (2006.01)

Patent

CA 2753546

Methods and compositions for increasing the starch content in cob tissues of a plant are provided. The method comprises down-regulating the activity of starch degradation enzymes in a plant. The resulting transgenic plants of the invention have increased starch content in cob tissues. In one embodiment the method involves manipulating a monocot plant to down-regu-late the activity of a starch degradation enzyme in cob tissues. The plants are useful for improving the yield of free sugars from plant biomass.

L'invention porte sur des procédés et des compositions pour augmenter la teneur en amidon dans les tissus verts d'une plante. Le procédé comprend la régulation négative de l'activité d'enzymes de dégradation de l'amidon dans une plante. Les plantes transgéniques ainsi obtenues de l'invention ont une teneur en amidon accrue dans les tissus verts et présentent un phénotype à excès d'amidon. Dans un mode de réalisation, le procédé met en jeu la manipulation d'une plante monocotylédone pour réguler de façon négative l'activité d'une enzyme de dégradation de l'amidon. Les plantes sont utiles pour améliorer la production de sucres libres à partir d'une biomasse végétale et augmenter la stabilité au stockage de tissu vert séché.

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