Method and apparatus for measuring environmental allergen

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/37 (2006.01) C12M 1/34 (2006.01) G01N 21/77 (2006.01) G01N 21/78 (2006.01)

Patent

CA 2568991

A method whereby an environmental allergen can be conveniently measured without resort to an antiallergen antibody and an instrument and an apparatus therefor. Namely, a method for measuring an environmental allergen with a biological origin which comprises measuring the protease activity of the allergen to thereby measure the allergen with a biological origin. An instrument for measuring an environmental allergen with a biological origin which is composed of a support and a substrate of a protease to be used for measuring the protease activity of the allergen carried on the support, wherein the substrate is a substrate causing, as the result of an enzyme reaction, generation of fluorescence, a change in absorbance or a color change.

La présente invention concerne un procédé et un appareil de mesure commode d'allergène environnemental sans recourir à un anticorps antiallergène. L'invention concerne plus particulièrement un procédé de mesure d'allergène environnemental d'origine biologique via une mesure de l'activité protéase de l'allergène. L'invention concerne également un instrument de mesure d'allergène environnemental d'origine biologique, composé d'un support et d'un substrat de protéase, s'utilisant pur mesurer l'activité protéase de l'allergène sur le support. En l'occurrence, suite à une réaction d'enzyme, le substrat provoque la génération d'une fluorescence, une modification de l'absorbance, ou un changement de couleur.

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