Improved method for identifying peptides in a biological sample

Details Improved method for identifying peptides in a biological sample Improved method for identifying peptides in a biological sample

A - Human Necessities

61

K

A61K 38/00 (2006.01)

Patent

CA 2563828

The invention provides a method for isolating a cargo peptide from a sample containing a cargo peptide-carrier protein complex. The method involves contacting a sample comprising a cargo peptide-carrier protein complex with a binding moiety selective for the carrier protein, under conditions wherein the carrier protein binds non-covalently to the binding moiety, and wherein the binding moiety is attached to a support, dissociating the cargo peptide from the cargo peptide-carrier protein complex, wherein the carrier protein remains bound to the binding moiety, and collecting the cargo peptide, whereby the cargo peptide is isolated from the sample.

L'invention concerne un procédé pour isoler un peptide cargo à partir d'un échantillon contenant un complexe de protéines porteuses et de peptides cargo. Ledit procédé implique la mise en contact d'un échantillon comprenant un complexe de protéine porteuse et de peptides cargo avec une fraction de liaison sélective pour la protéine porteuse, dans des condition, dans lesquelles la protéine porteuse lie de manière non covalente la fraction de liaison, la fraction de liaison étant fixée au support, dissociant le peptide cargo du complexe de protéine porteuse-peptide cargo, la protéine porteuse restant liée à la fraction de liaison. Ledit procédé comprend également la collecte du peptide cargo, ce dernier étant isolé de l'échantillon.

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