Methods for characterising mrna molecules

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2322068

The invention relates to methods for the qualitative and quantitative determination of differentially expressed mRNA molecules. Said methods are used especially to determine if possible all mRNA molecules present in a cell or a tissue, and to compare them with other cells or tissues or with other conditions (stages of disease or development) or stages of treatment for same. The method provided for in the invention therefore makes it possible, for example, to establish a comprehensive map of the different mRNA molecules present in a defined mRNA population and subsequently to use the preferably digital information obtained in this way in data base analyses.

L'invention concerne des procédés pour la détermination qualitative et quantitative de molécules d'ARNm exprimées de manière différentielle. Ces procédés servent en particulier à la détection, si possible, de toutes les molécules d'ARNm présentes dans une cellule ou un tissu, ainsi qu'à leur comparaison avec d'autres cellules ou tissus ou avec d'autres états (stades de maladies ou de développement) ou phases de traitement de ces derniers. Les procédés de l'invention permettent ainsi d'établir par exemple une représentation complète des différentes molécules d'ARNm présentes dans une population d'ARNm définie, et d'utiliser ensuite les informations, de préférence numériques, ainsi obtenues, dans le cadre d'analyses de banques de données.

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