Assay for detection of transferase enzyme activity in drug...

Details Assay for detection of transferase enzyme activity in drug... Assay for detection of transferase enzyme activity in drug...

C - Chemistry, Metallurgy

12

Q

C12Q 1/48 (2006.01) C12Q 1/18 (2006.01) G01N 33/53 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2407934

The invention provides methods for assaying the activity of the translocase enzyme and/or transferase enzyme involved in peptidoglycan biosynthesis in bacteria using scintillation proximity assay methodology. The methods are suitable for high throughput screening of potential anti-bacterial drugs.

L'invention concerne des procédés permettant de tester l'activité de l'enzyme translocase et/ou l'enzyme transférase impliquées dans la biosynthèse de peptidoglycane de bactéries selon par un processus de dosage de la scintillation par proximité. Les procédés conviennent au tri haut à rendement de drogues antibactériennes potentielles.

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