Amplifiable probe

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01)

Patent

CA 2449911

The invention relates to an amplifiable probe comprising: (a) an analyte reactive moeity; (b) at least one signal-generating nucleic acid, wherein each said at least one signal-generating nucleic acid further comprises at least one self-complementary unit, wherein each said at least one self-complementary unit comprises a first sequence element and a second element, and wherein said first and said second sequence elements are complementary; and (c) one or more linkers that connect said analyte reactive moiety to said at least one said signal-generating nucleic acid. The probe may be used in methods for detecting analytes such as polypeptides, nucleic acids, carbohydrates, lipids or haptens. The probes contain an analyte reactive moiety linked to a nucleic acid molecule that can be extended by DNA polymerase.

L'invention concerne une sonde amplifiable comprenant: (a) une partie sensible aux analytes, (b) au moins un acide nucléique générateur de signaux, chaque acide nucléique générateur de signaux comportant également au moins une unité auto-complémentaire, chaque unité présentant un premier élément de séquence et un second élément de séquence et chaque élément de séquence étant complémentaire, ainsi que (c) un ou plusieurs lieurs reliant ladite partie sensible aux analytes à au moins un acide nucléique générateur de signaux. Cette sonde peut être utilisée dans des procédés de détection d'analytes tels que des polypeptides, des acides nucléiques, des carbohydrates, des lipides ou des haptènes. Ces sondes contiennent une partie sensible aux analytes liée à une molécule d'acide nucléique qui peut être allongée par l'ADN polymérase.

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