Highly conserved genes and their use to generate probes and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 14/00 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2388461

Four highly conserved genes, encoding translation elongation factor Tu, translation elongation factor G, the catalytic subunit of proton-translocating ATPase and the RecA recombinase, are used to generate a sequence repertory or bank and species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes and amplification primers to rapidly detect and identify algal, archaeal, bacterial, fungal and parasitical microorganisms from specimens for diagnosis. The detection of associated antimicrobial agents resistance and toxin genes are also under the scope of the present invention.

Cette invention se rapporte à quatre gènes à fort pouvoir de conservation, le facteur d'élongation de traduction codant Tu, le facteur d'élongation de traduction G, la sous-unité catalytique de l'enzyme ATPase de translocation de protons et la recombinase RecA, qui sont utilisés pour produire un répertoire ou une banque de séquences et des sondes d'acide nucléique spécifiques à l'espèce, spécifiques au gène, spécifiques à la famille, spécifiques au groupe et universelles et des amorces d'amplification, en vue de détecter et d'identifier rapidement des micro-organismes algaires, archéobactériens, bactériens, fongiques et parasitaires contenus dans des spécimens, à des fins de diagnostic. La détection des gènes de toxine et de résistance aux agents antimicrobiens associés entre également dans le cadre de cette invention.

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