Calibration method for automated assay instrument

G - Physics – 01 – N

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G01N 35/10 (2006.01) G01N 35/06 (1990.01)

Patent

CA 2070401

-43- 7671 ABSTRACT OF THE DISCLOSURE A method of calibrating pipette position in an automated assay instrument employing a conveyor for holding assay cartridges and a transport for transporting a pipette between various locations is accomplished by alignment of a pipette stem with targets located at various locations within the system. Two targets are located on a tray having a plurality of reservoirs for holding liquid, and one target is located on a dummy cartridge carried by the conveyor. An indicator having a spring-loaded tab is used in conjunction with a pipette stem modified by inclusion of a circumferential slot for receiving the spring loaded tab. The stem is inserted into the indicator and locked therein, with limited sliding being provided by movement of the tab within confines of the slot. Alignment marks are provided on both the stem and the indicator to permit visual alignment of the stem relative to a target upon abutment of the indicator with the target. The height of the indicator along with the displacement of the stem from a reference position is inserted into a microprocessor of the assay instrument.

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