Non-hemolytic clya for excretion of proteins

Details Non-hemolytic clya for excretion of proteins Non-hemolytic clya for excretion of proteins

C - Chemistry, Metallurgy

07

K

C07K 19/00 (2006.01) C12N 15/64 (2006.01)

Patent

CA 2726293

The disclosure below provides a protein export system utilizing non-hemolytic variants of HlyE family member proteins for efficiently producing recombinant protein from a host cell. In a preferred embodiment, the protein export system uti-lizies protein export machinery endogenous to the host bacterium into which the protein export system vector is introduced.

L'invention porte sur un système d'export de protéine utilisant des variantes non hémolytiques de protéines membres de la famille HlyE pour la production efficace d'une protéine recombinante à partir d'une cellule hôte. Dans un mode de réalisation préféré, le système d'export de protéine utilise la machinerie d'export de protéine endogène à la bactérie hôte dans laquelle le vecteur de système d'export de protéine est introduit.

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